rabbit anti-human cd68 polyclonal ab  (Millipore)

Journal: Cell

Article Title: In vitro and in vivo functions of SARS-CoV-2 infection-enhancing and neutralizing antibodies

doi: 10.1016/j.cell.2021.06.021

Figure Lengend Snippet: Lung histopathology of Ab-treated and SARS-CoV-2 challenged cynomolgus macaques, related to and (A) Representative images of hematoxylin and eosin (H&E) staining and SARS-CoV-2 antigen immunohistochemistry (IHC) staining from each group. All images were taken at 10x magnification. The images in this figure are representative of the average severity of pathologic processes observed and recorded during microscopic evaluation. Red arrows indicate SARS-CoV-2 infection foci. (B) Following microscopic evaluation of DH1052, 1 animal (BB536A) out of 5 animals in this group exhibited histologic features that was substantially more severe than the rest of the cohort and may suggest some degree of Ab-mediated disease enhancement. The features were characterized by prominent perivascular mononuclear inflammation ( ∗ ) and a substantial amount of perivascular and alveolar edema (fluid; X). These findings suggest a vaso-centric process with some degree of altered vascular permeability. The remaining 4 animals in DH1052 group had inflammatory changes that ranged from minimal to moderate severity and more infiltrates were mixed and predominantly polymorphonuclear with lesser mononuclear cell involvement and present in the alveolar spaces. (C-E) Expression of macrophage activation markers in macaque lung tissues. An animal from the CH65 control group (C), the DH1052-treated animal (BB536A) that exhibited substantially more severe lung inflammation (D), and an animal from the NTD NAb DH1050.1 group (E) were selected for Immunohistochemistry (IHC) staining. Immunohistochemical staining was performed using MHCII, CD68, IBA1 and CD163 to detect classically activated macrophages (M1) and/or alternatively activated macrophages (M2). CD11b is a macrophage/monocyte marker and CD3 is a T cell marker. All images are 10x magnification; scale bars = 100μm.

Article Snippet: Rabbit anti-human CD68 polyclonal Ab , Sigma-Millipore , Cat# HPA048982, RRID: AB_2680587.

Techniques: Histopathology, Staining, Immunohistochemistry, Infection, Permeability, Expressing, Activation Assay, Control, Immunohistochemical staining, Marker

Journal: Cell

Article Title: In vitro and in vivo functions of SARS-CoV-2 infection-enhancing and neutralizing antibodies

doi: 10.1016/j.cell.2021.06.021

Figure Lengend Snippet:

Article Snippet: Rabbit anti-human CD68 polyclonal Ab , Sigma-Millipore , Cat# HPA048982, RRID: AB_2680587.

Techniques: Virus, Clinical Proteomics, Recombinant, Staining, Reverse Transcription, Random Hexamer, Luciferase, Cell Culture, Lysis, Membrane, Multiplex Assay, Reporter Gene Assay, Binding Assay, Expressing, Transgenic Assay, Sequencing, Software

Journal: Frontiers in cellular and infection microbiology

Article Title: Dietary Arginine Regulates Severity of Experimental Colitis and Affects the Colonic Microbiome.

doi: 10.3389/fcimb.2019.00066

Figure Lengend Snippet: FIGURE 4 | Regulation of NOS2 and arginase-1. NOS2, arginase-1, and CD68 were immunodetected in colonic tissues from C57BL/6 mice ± DSS or C. rodentium ± Arg0, ArgNL, or ArgHIGH diets. In each panel, CD68 is depicted in red, NOS2 (A) or arginase-1 (B) in green, and the nuclei in blue; CD68+NOS2+ and CD68+ARG1+ cells are shown in yellow. The data shown are representative photomicrographs of at least 3 animals per condition. Scale bar, 50 µm.

Article Snippet: Immunofluorescent staining for NOS2, arginase-1 and the macrophage marker CD68 was performed on the colon tissues as described (Singh et al., 2018) using a rabbit polyclonal antiNOS2 (Novus Biological; 1/100), a goat polyclonal anti-arginase1 (Santa Cruz; 1/100), and a rabbit polyclonal anti-mouse CD68 Ab (Boster Biological, 1/100), respectively.

Techniques: